HPLC Columns and Cartridges
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TSKgel™ CM-3SW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ CM-3SW columns are packed with 5 micron, high efficiency, spherical silica beads which are surface modified with a weak cation exchanger. They are used for the separation and analysis of proteins, peptides, and other biologically active molecules. They have a larger pore size than the TSKgel™ CM-2SW columns.
Thermo Scientific™ BioBasic™ 8 HPLC Columns
Use this column for reversed-phase separation of proteins and other biomolecules.
| Pore Size | 300 Å |
|---|---|
| Particle Size | 5 μm |
| Carbon Load | 5% |
| Temperature | 60°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| pH | 2 to 9 |
| Surface Area | 100 m²/g |
| Max. Pressure | 5800 psi (400 bar) |
| Product Line | BioBasic |
| USP Type | L7 |
Thermo Scientific™ ProPac™ PA1 HPLC Columns
Efficiently analyze and purify hydrophilic anionic proteins and peptides with ProPac™ PA1 LC Columns.
| Pore Size | Nonporous |
|---|---|
| Particle Size | 10 μm |
| Flow Rate | 0.9 to 1.5 mL/min |
| Stationary Phase | Alkyl Quaternary Amine |
| Phase | Ion Exchange |
| For Use With | BioLC Chromatography System |
| Packing Material | Spherical, Nonporous Polymer |
| Column Type | Ion Exchange |
| Quantity | Each |
| Green Features | FSC |
| Material | PEEK |
| Max. Pressure | 5000 psi (345 bar) |
| Product Line | ProPac |
MilliporeSigma™ LiChroCART™ Columns/Purospher™ STAR RP-8 Endcapped Sorbents HPLC Cartridges, 5μm
Optimized for more polar compounds
| Pore Size | 120 Å |
|---|---|
| Stationary Phase | C8 |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| Quantity | 1/Pk. |
| Surface Area | 330 m²/g |
| Max. Pressure | 2175 psi (150 bar) to 2900 psi (200 bar) |
| Column Format | Cartridge |
| Particle Size | 5 μm |
| Carbon Load | 17% |
| Product Type | Cartridge |
| Packing Material | Silica |
| pH | 1.5 to 10.5 |
| USP Type | L7 |
Thermo Scientific™ Hypersil™ Phenyl-2 HPLC Columns
Use as a general-purpose column with aromatic selectivity with low silanol binding.
| Pore Size | 120 Å |
|---|---|
| Particle Size | 5 μm |
| Carbon Load | 5% |
| Temperature | 60°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| pH | 2 to 8 |
| Surface Area | 170 m²/g |
| Max. Pressure | 5800 psi (400 bar) |
| Product Line | Hypersil Classical |
| USP Type | L11 |
MilliporeSigma™ Supelco™ Ascentis™ Express 90 Å PAH, 2.7 μm HPLC Guard Cartridge
The unendcapped trifunctional C18 stationary phase of Ascentis™ Express PAH provides a stable, reversed-phase packing that can be used for analysis of acids, bases, and zwitterionic compounds, but has been specifically designed for the separation of polycyclic aromatic hydrocarbons.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2.7 μm |
| Temperature | <60°C |
| Matrix | Fused-Core™ Silica Particle Platform Particle Platform Superficially Porous Particle |
| Length (Metric) | 0.5 cm |
| Column Type | Reversed Phase |
| pH | 2 to 9 |
| Quantity | 3/Pk. |
| Material | Stainless Steel |
| Surface Area | 135 m2/g |
| Max. Pressure | 600 bar |
TSKgel™ ODS-80Ts HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel ODS-80Ts is a general purpose column for low MW pharmaceuticals, bases, nucleosides, and nucleotides. Common applications include purity checks and peptide mapping.
TSKgel™ SuperH4000 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Packed with rigid, porous polymer beads and used for the non-aqueous separation of polymers with molecular weights less than 400,000 Da.
TSKgel™ Chelate-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™
Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, loaded with divalent metal ions by chelation.
| Solvents | ≤ 20% Organic Solvent |
|---|---|
| Stationary Phase | Iminodiacetic Acid |
| Temperature | ≤45°C |
| Matrix | Iminodiacetic Acid Phase/Hydroxylated Methacrylic Polymer |
| pH | 2 to 12 |
| Technique | Affinity |
| Product Line | TSKgel |
TSKgel™ SuperHZ2500 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel SuperHZ2500 Columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 20,000 Da.
| Pore Size | 30 Å |
|---|---|
| Particle Size | 3 μm |
| Stationary Phase | Polystyrene-divinylbenzene |
| Temperature | 60°C |
| Matrix | Polystyrene-divinylbenzene Phase/Polymer Particle Platform |
| Length (Metric) | 15 cm |
| Material | Stainless Steel Hardware |
| Technique | SEC |
| Max. Pressure | 40 bar |
| Product Line | TSKgel |
TSKgel™ ODS-120A Guard Cartridge, Sold by MilliporeSigma™ Supelco™
for 4.6 mm ID TSKgel ODS-120A columns
MilliporeSigma™ Supelco™ Ascentis™ Express C8 HPLC Column, 2.7 μm Particle Size
Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2.7 μm |
| Stationary Phase | C8 (Octyl) |
| Phase | Reversed Phase |
| Matrix | C8 (Octyl) Phase/Fused-Core Particle Platform |
| Endcapped | Yes |
| pH | 2 to 9 |
| Technique | Fused-Core HPLC |
| Product Line | Ascentis |
MilliporeSigma™ Supelco™ SUPELCOSIL™ Suplex™ pKb-100 HPLC Column, 5 μm Particle Size
Suplex™ pKb-100 columns feature the same bonded phase functionality as SUPELCOSIL™ LC-ABZ columns.
| Pore Size | 120 Å |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | Amide, Alkyl |
| Phase | Reversed Phase |
| Matrix | Amide, Alkyl Phase/Silica Gel/Spherical Particle Platform |
| Endcapped | No |
| Surface Area | 170 m2/g |
| Product Line | Suplex |
TSKgel™ ODS-120A HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ ODS-120A columns use a 150 Å pore size silica base support. The silica′s exclusion limit of 10,000 Da makes them a good choice for the reversed phase chromatography of peptides, small proteins, and environmental samples such as poly-aromatic hydrocarbons.
| Pore Size | 150 Å |
|---|---|
| Solvents | 0 to 100% organic solvent |
| Stationary Phase | C18 (octadecyl) |
| Phase | Reversed Phase |
| Endcapped | No |
| Surface Area | 200 m2/g |
| Particle Size | 5 μm |
| Flow Rate | 1.2 mL/min. |
| Carbon Load | 20% |
| Matrix | Silica Particle Platform |
| Diameter (Metric) | 4.6 mm |
| pH | 2.0 - 7.5 |
| Product Line | TSKgel |
| USP Type | L1 |
Thermo Scientific™ POROS™ CaptureSelect™ HSA Affinty HPLC Columns
Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.
| Shipping Condition | Wet Ice |
|---|---|
| Pore Size | 2000 Å |
| Stationary Phase | Human Serum Albumin affinity ligand, humanspecific |
| Column Type | Affinity |
| Quantity | 1 Column |
| Material | PEEK |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Max. Pressure | 180 bar |
| Particle Size | 20 μm |
| Flow Rate | Up to 5000 cm/hr. |
| Matrix | Polystyrenedivinylbenzene |
| Reactivity | Human |
| Target Molecule | HSA, HSA Fusion Proteins |
| Product Type | Column |
| Binding Capacity | 2.6 mg/mL |
| Sample Type | Cell Culture Supernatants |
| Purification Target | HSA, HSA Fusion Proteins |
| Product Line | CaptureSelect, POROS |